Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-01-04
2010-06-01
Sisson, Bradley L (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S173700, C435S270000, C436S094000, C536S023100
Reexamination Certificate
active
07727718
ABSTRACT:
The present invention describes reagents and methods for storing and/or processing of biological samples for direct use in PCR and other DNA applications. After storage, the preserved DNA in the samples may then be processed and analyzed by known methods, e.g., PCR. the present invention provides in one aspect, a method for simple and rapid storage and/or processing of nucleic acids, such as DNA, from various sources, including but not limited to body fluids, various solutions, cells, plants, tissues, bacterial cell lysates containing plasmids, etc. The present invention further comprises reagents and methods employing glycols at alkaline pH to process biological samples and make DNA usable in PCR without further sample purification. Accordingly, the present invention provides in one aspect, a method for simple and rapid processing of nucleic acids, such as DNA, from various sources, including but not limited to body fluids, various solutions, cells, plants, tissues, bacterial cell lysates containing plasmids, etc.
REFERENCES:
patent: 5346994 (1994-09-01), Chomczynski
patent: 5945515 (1999-08-01), Chomczynski
patent: 6469159 (2002-10-01), Belly et al.
patent: 6831050 (2004-12-01), Murch et al.
patent: 2002/0168361 (2002-11-01), Kelly
patent: 2003/0149097 (2003-08-01), Beilfuss et al.
patent: 2003/0170822 (2003-09-01), Itoh
patent: 2004/0115131 (2004-06-01), Hellerstein
patent: WO 8900577 (1989-01-01), None
Ashen, N., Oellerich M. and Schutz E, “Use of two reporter dyes without interference in a single-tube rapid-cycle PCR: α1-antitrypsin genotyping by multiplex real time fluorescence PCR with the LightCycler” 2000, Clin. Chem. 46, 156-161.
Camoes I.C.G., Salles M.R., Chevitarese O., and Gomes G.C. “Influence of pH vehicle containing glycerin used with calcium hydroxide” 2003, Dental Traumatology 19, 132-138.
FTA Cards, Cat No. 10786-010, Invitrogen, Carlsbad, CA.
Joshi A.K., Baichwal V. and Ames G.F. “Rapid polymaerase chain reaction amplification using intact bacterial cells” 1991, Biotechniques 10, 42-44.
Leal-Klevezas D.S., Martinez-Vazquez I.O., Cuveas-Frenandez B. and Martinez-Soriano J.P. “Antifreeze solution improves recovery by preserving the integrity of pathogen-infected blood and other tissues” 2000, Clinical and Diagnostic Laboratory Immunology 7, 945-946.
Lin Z. and Floros J. “Protocol for genomic DNA preparation from fresh or frozen serum for PCR amplification” 2000, Biotechniques 29, 460-466.
Mercier, B. Gaucher, C. Feugeas, O. and Mazurier C. “Direct PCR from whole blood, without DNA extraction”, 1990, Nucl. Acids Res. 18, 5908.
Moore, David and Dowhan, Dennis, “Purification and Concentration of DNA from Aqueous Solutions,”Current Protocols in Molecular Biology(2002) 2.1.1-2.1.10 (2002) John Wiley & Sons, Inc.
Ohhara M, Kurosu Y. and Esumi M. “Direct PCR of whole blood and hair shafts by microwave treatment” 1994, Biotechniques 17, 726-728.
Rogers C. and Burgoyne L. “Bacterial typing” 1997, Anal. Biochem. 247, 223-227.
Rudbeck L and Dissing J “RAPID, Simple alkaline extraction of human genomic DNA from whole blood, buccal epithelial cells, semen and forensic stains for PCR” 1998, Biotechniques 25, 588-592.
Sandhu G.S., Precup J.W. and Kline B.C. “Rapid One-step characterization of recombinant vectors by direct analysis of transformedEscherichia colicolonies” 1989, Biotechniques 7, 689-690.
Subbarayan P.R. Sarkar M. and Ardalan B. “Isolation of genomic DNA from human blood” 2002, Biotechniques, 1231-1234.
Truett G.E., Heeger R.L., Mynatt A.A. Truett J.A., Walker J.A. and Warman M.L. “Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris” 2000, Biotechniques 29, 52-54.
Frost Brown Todd LLC
Molecular Research Center, Inc.
Sisson Bradley L
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