Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-03-19
1998-01-20
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 792, 435 793, 435 794, 435 795, 436 72, 436175, 536 221, C12Q 168, G01N 3353, G01N 3300
Patent
active
057100063
ABSTRACT:
A sensitive assay method has been discovered that reduces the amount of non-specific binding present in an assay, the method comprising detecting an analyte present in a sample through a specific binding reaction in which either an analogue of the analyte or a specific binding partner of the analyte is immobilized on a solid-phase and said specific binding reaction produces a detectable product immobilized on said solid-phase that may be correlated to the amount of analyte present in the sample wherein said assay employs an effective amount of a surfactant selected from the group consisting of a polyoxyethylene-alkylether, a polyalkylene oxide-modified polydimethylsiloxane block copolymer, a polyalkylene oxide-modified polymethylsiloxane block copolymer, and mixtures thereof to reduce non-specific binding.
REFERENCES:
patent: 4281061 (1981-07-01), Zuk et al.
patent: 4668639 (1987-05-01), Johannsson
patent: 4810630 (1989-03-01), Craig et al.
patent: 4927769 (1990-05-01), Chang et al.
patent: 4971904 (1990-11-01), Luddy
patent: 5248620 (1993-09-01), Sluka et al.
Haycock, John W., 208 Analytical Biochemistry, 397-399 (1993).
Kenna, J. G. et al., 85 J. Immunological Methods, 409-419 (1985).
Mohammad, Kamaruzaman et al., 117 J. Immunological Methods 141-145 (1989).
OSI Specialties, SILWET.RTM. Surfactants (Dec. 9, 1986).
Papavassiliou, Athanasios G. et al., 20 Nucleic Acids Research, 4365-4366 (1992).
Reeves, Stuart G. et al. 26 (7) Analytical Letters 1461-1476 (1993).
Weeks, Ian et al., 29/8 Clin. Chem. 1474-1479 (1983).
Kiaei David
Livshin Laurie A.
Piran Uri
Blackburn Robert P.
Chiron Diagnostics Corporation
Housel James C.
Morgenstern Arthur S.
Shaver Jennifer
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