Reagents for diagnosing, imaging and treating...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C436S512000, C436S547000, C435S007210, C435S070210, C424S130100, C530S300000, C530S350000, C530S387100

Reexamination Certificate

active

07575873

ABSTRACT:
The invention provides a novel human Mab Fab, cloned by phage display, and its use in diagnostic and therapeutic methods. In particular the invention provides a method for analyzing the OxLDL components of atherosclerotic plaques in vivo and a means to determine their relative pathology. As the method is based on a human Fab rather than a mouse Mab, the progress or regression of the disease may be monitored over time. The antibody may also be used for the analysis of surgical or serum samples ex vivo for the presence of OxLDL. The antibody may also be used to target therapeutic agents to the site of atherosclerotic plaques or may have use as a therapeutic agent itself.

REFERENCES:
patent: 5330910 (1994-07-01), Young et al.
patent: 5460947 (1995-10-01), Young et al.
patent: 6225070 (2001-05-01), Witztum et al.
patent: 6375925 (2002-04-01), Witztum et al.
patent: WO 94/23302 (1994-10-01), None
patent: WO/98/21581 (1998-05-01), None
Sotiriadou et al., DELTA-IK17: An antigen expressed on human lymphocytes. International Journal of BiologicalMarkers, 1996, vol. 11, No. 4, pp. 183-189.
Jein et al., J Med Chem., 1994, 37, 2100-2105.
Kuduk et al. Bio & Med Chemistry Letters, 10, 2000, 1303-1306.
Peipp and Valerius, Biochemistry Society Transactions, 2002, vol. 30, part 4, pp. 507-511.
Waldmann, Science, vol. 252, Jun. 21, 1991, pp. 1657-1662.
Hoorko S. Miller, E. Dudl E. et al Antiphospholipid antibodies are directed against epitopes of oxidized phospholipids:recognition of cardiolipin by monoclonal antibodies to epitopes of oxidized LDL. J. Clin. Invest.;1996:98; 815-825.
Huse WD, Sastry L, Iverson SA, Klang AS, Alting-Mees M. Burton DR, Benkovic SJ, Lerner RA. Generation of a large combinatorial library of immunoglobulin repertoire in phage lambda. Science. 1989;246:1275-1281.
Palinski W. Horkko, S. Miller et al. Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins for apo E-deficient mice. Demostration of epitopes of oxidezed LDL in human plasma. J.Clon.Invest 1996;98:800-814.
Shaw P.X, Horkko, S. Chang, M-K Curtiss, L.K. Palinski, W. Silverman, G.J. and Witztum, J.L. Natural antibodies with T15 Idiotype may act in atheosclerosis, apoptotoc clearance and protective immunity. J. Clin, Invest. 105:1731-40, 2000.
Wu CC, Chang SW, Chen MS, Lee YT. Early change of vascular permeability in hypercholoesterloemic rabbits. Arterioscler Thomb Vasc Biol. 1995, 15-529-533.
Libby P. et al. Stabilizing of atherosclerotic plaques: New mechanisms and clinical targets. Nature Medicine. vol. 8, No. 11, 1257-1262-Nov. 2002.
Narula, J. et al. Strategic targeting of atherosclerotic lesions. Journal of Nucelar Cardiology, Part 1, pp. 81-90 Jan./Feb. 1999.
Strauss, H.W. Imaging of atheosclerosis—A worthy challenge. Journal of Nucelar Cardiology, vol. 3, No. 3, pp. 278-280 May/Jun. 1996.
Tsimikas, S. et al. In vivo uptake of Radiolabeled MDA2, an oxidation-specific monoclonal antibody provides an accurate measure..Arterioscler Thromb, Vasc. Biol., vol. 20:689-697.2006.
Holovet, P. et al, Monodialdehyde-modified low density lipoproteins in patients with atherosclerotic disease. J. Clin Invest. vol. 95:2611-9. 1995.
Tsimikas S. Palinski W, Halpern SE, Yeung DW, Curtiss LK, Witztum JL. Radiolabeled MDA2, an oxidation-specific Mab, identifies native atherosclerotic lesions in vivo. J. Nucl. Cardiol. 1999; 6:41-53.
Barbas, CF, et al., “Combinatorial Immunoglobulin Libraries on the Surface of Phage (pphabs): Rapid Selection of Antigen-Specific Fates,” METHODS: A Companion to Methods in Enzymology, 1991, 2(2): 119-124, Academic Press, Inc., Burlington, MA.
Chang, M-K, et al., “Monoclonal Antibodies Against Oxidized LDL Bind to Apoptotic Cells and Inhibit Their Phagocytosis by Elicited Macrophages: Evidence That Oxidation Specific Epitopes Mediate Macrophage Recognition,” Proc. Nat. Acad. Sci. U.S.A., 1999, 96:6353-.
van der Kooij, MA, et al., “Human Monocyte—Derived Macrophages Express and Approximately 120-kD Ox-LDL Binding Protein With Strong Identity to CD 68”, 1997, 17(11): 3107-3116.

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