Reagents for detecting and assaying nucleic acid sequences

Details Reagents for detecting and assaying nucleic acid sequences Reagents for detecting and assaying nucleic acid sequences

1986-09-10

1992-12-29

Yarbrough, Amelia Burgess

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 91, 435291, 436501, 436811, 536 28, 536 29, 935 32, 935 78, C07H 2100, C12Q 168

Patent

active

051752700

ABSTRACT:
A novel class of reagents for assaying nucleic acid sequences comprise successive layers of polynucleotides of a specific structure, including a double-stranded waist and single-stranded, free arms at the molecule ends, formed by hybridization of the arms to adjacent molecule arms. The reagent is used to assay for specific nucleic acid sequences. The outer layer of polynucleotides are specific for the sequence to be assayed through their non-annealed, free, single-stranded arms. The reagents are useful in the assay of a wide variety of nucleic acid sequences including those associated with pathogens such as pathogenic bacteria and virus.

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Cech (1981) Biochemistry, vol. 20, pp. 1431-1437.
Worthy, "New Families of Multibranched Macromolecules", C&EN, 19-21 (Feb. 22, 1988).
S. B. Zimmermann et al., "Helical parameters of DNA do not change when DNA fibers are wetted: X-ray diffraction study," Proc. Natl. Acad. Sci. USA, vol. 76, pp. 2703-2707 (1979).
V. D. Osica et al., "Preliminary morphological and X-ray diffraction studies of the crystals of the DNA Cetyltrimethylammonium Salt," Nucleic Acids Research, vol. 4, No. 4, pp. 1083-1096 (Apr. 1977).

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