Reagents for CO.sub.2 detection

Details Reagents for CO.sub.2 detection Reagents for CO.sub.2 detection

1990-07-20

1992-05-26

Nucker, Christine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 17, 435 12, 435 25, 435 26, 436 8, 436 9, 436 18, 436 19, C12Q 154, C12Q 158, C12Q 150, C12Q 126

Patent

active

051167280

ABSTRACT:
A stabilizer for maintaining a constant level of CO.sub.2 in coenzyme containing enzymatic CO.sub.2 diagnostic reagents is disclosed, comprising rate-limiting amounts of (a) the diagnostic reagents and (b) coenzyme-regenerating reagents, e.g., wherein (a) is malate dehydrogenase, phosphoenolpyruvate carboxylase and NADH, and (b) is glucose dehydrogenase and glucose, as are methods of use and kits containing a diagnostic reagent and stabilizer.

REFERENCES:
patent: 4271264 (1981-06-01), Modrovich
patent: 4277562 (1981-07-01), Modrovich
patent: 4394449 (1983-07-01), Modrovich
Chenault et al., 1987, Review: Regeneration of Nicotinamide Cofactors for Use in Organic Synthesis Appl Biochem. Biotech. 14:147-197.
Tietz, ed., Textbook of Clinical Chemistry (W. B. Saunders Company: Philadelphia), pp. 1800-1801.
Wu et al., Clin. Chem. 32, 314-319 (1986).
Chenault et al., Appl Biochem. Biotech. 4, 147-197 (1987).
Morris et al., Clin. Chem. 36, 131-135 (1990).
Konopka et al., Clin. Chem. 36, 1068 (1990).

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