Reagents for assay of gamma-glutamyltranspeptidase

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

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435 16, 435 24, 435 26, 435 711, C12Q 148, C12Q 152, C12P 2102

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active

051262452

ABSTRACT:
A reagent for assaying .gamma.-glutamyltranspeptidase (.gamma.-GTP) which contains an amino acid dehydrogenase and a substrate specific to .gamma.-glutamyltranspeptidase activity. The reagent can be used to assay the .gamma.-GTP activity directly from the continuous course of reaction without using a standard substance with known activity. The procedure required is simple and easy, and the reagent is capable of determining .gamma.-GTP activity with good accuracy and without being affected by interfering substances, making it satisfactory for routine use in clinical laboratories.

REFERENCES:
patent: 4024021 (1977-05-01), Stavropoulos et al.
patent: 4511651 (1985-04-01), Beaty et al.
International Union of Biochemistry, 1978, Enzyme Nomenclature, pp. 158-159, No. 2.3.2.2, Academic Press, New York.
Lehninger, Biochemistry, 2nd Edition, 1975 p. 795.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, vol. 34, No, 4, Oct. 1960, pp. 381-398, US; R. J. Henry et al. "Revised spectrophotomertic methods for the determination of gluamic-oxalacetic transaminase, glutamic-pyruvic transaminase, and lactic acid dehydrogenase".

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