Optics: measuring and testing – By shade or color – With sample responsive to plural colors applied simultaneously
Reexamination Certificate
2007-11-07
2008-11-04
Punnoose, Roy M (Department: 2886)
Optics: measuring and testing
By shade or color
With sample responsive to plural colors applied simultaneously
Reexamination Certificate
active
07446874
ABSTRACT:
A formulation for preparing water samples for determining arsenic concentration by colorimetry is provided. The chemical reagents in the formulation may be provided as premeasured and premixed tablets or pills. The chemical reagents include sulfamic acid, an oxidizer, and color reagents for selectively forming molybdenum-based color complexes with arsenates and phosphates. To test for arsenic, the water samples are acidified by addition of sulfamic acid. Sulfamic acid also advantageously reduces arsenic to an arsenite state. Arsenic in the water sample is oxidized or re-oxidized to an arsenate state by addition of the oxidizer. Then, color reagents are used to selectively bind and convert arsenates and phosphates in the water samples into molybdenum-blue color complexes. The light absorbance of the water sample with both arsenates and phosphates bound in molybdenum-blue color complexes is compared to that of a reference acidified specimen in which mostly only the phosphates but not the arsenates are bound and converted. The differential light absorbance of the two specimens is used to arrive at a quantitative value for the arsenic concentration in the water sample.
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Johnson, D. L., “Simultaneous determination of arsenate and phosphate in natural waters,” Environmental Science and Technology, 5, pp. 411-14, 1971.
Baker & Botts LLP
Punnoose Roy M
The Trustees of Columbia University in the City of New York
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