Reagents containing an anti-ligand bound to an anti-enzyme and m

Details Reagents containing an anti-ligand bound to an anti-enzyme and m Reagents containing an anti-ligand bound to an anti-enzyme and m

1984-03-12

1986-11-04

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 7, 435 15, 435 17, 435 18, 435 21, 435 26, 435188, 436512, 436513, 436537, 436548, 436813, 436817, 436822, 530389, G01N 33536

Patent

active

046210482

ABSTRACT:
The invention relates to a method and novel reagents for detecting a ligand in a biological sample. In the method, the sample is combined with (i) an enzyme or an enzyme bound to a first macromolecular compound, (ii) substrate for the enzyme, and (iii) an antibody complex comprising anti-ligand bound to anti-enzyme and a second macromolecular compound bound to either one or both of the anti-ligand and anti-enzyme. The activity of the enzyme varies as a function of the unknown amount of ligand contained in the biological sample.

REFERENCES:
patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4433059 (1984-02-01), Chang et al.
patent: 4446233 (1984-05-01), Auditore-Hargreaves et al.

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