Reagents and procedures for high-specificity labeling

Details Reagents and procedures for high-specificity labeling Reagents and procedures for high-specificity labeling

2006-11-28

2006-11-28

Ceperley, Mary E. (Department: 1641)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Chemical modification or the reaction product thereof, e.g.,...

C436S172000, C436S800000, C530S408000, C530S409000, C530S410000, C548S102000, C548S402000

Reexamination Certificate

active

07141655

ABSTRACT:
A molecule with two pendant phenylarsine moieties according to the general structural Formula (I) and tautomers, acids, and salts thereof:wherein: (i) R1or R2, are each independently O−, S−, OR3or SR3with the provision that if either R1or R2is absent, the other remaining group is ═O or ═S; or R1and R2, together with the arsenic atom, form a ring according to one of the general structural Formulae (II), (III), (IV), or (V):wherein R3is H, CH(OH)CH2OH, or (CH2)q—Y, with q being 1–4 and Y being H, OH, NH2, SH, COOH, OAc, CONH2or CN, and Z represents a hydrocarbon chain comprising 2–4 singly or doubly bonded carbon atoms each of which may be further substituted with one or more of hydrogen, methyl, ethyl, 1-propyl, 2-propyl, methoxy, hydroxy, amino, carboxy, sulfo, oxo, thio, halo (fluoro, chloro, bromo, or fluoro) and (CH2)n″SO3, wherein n″ is 1 or 2; (ii) R4, R5, R6and R7are each independently H, F, OR3, R3, OAc, NH2, N(C1–C4alkyl)2, R1; or R4with R5, or R6together with R7, or both, form a ring; (iii) R8is a linear or branched optionally substituted spacer having a minimum length of approximately 1.5 and a maximum length of approximately 15 Ångstroms; and (iv) X is a detectable group. Methods of using the bis-phenylarsine molecule also are provided.

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