Reagents and methods for the quantification of total doxepins in

Details Reagents and methods for the quantification of total doxepins in Reagents and methods for the quantification of total doxepins in

1994-04-12

1995-11-07

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

530404, 530405, 530406, 540550, 544212, 549223, C12N 996, C07K 1476, C07D26716, C07D31180

Patent

active

054647673

ABSTRACT:
Immunoassay methods and reagents for the quantification of total doxepins (i.e., E-doxepin, Z-doxepin, E-desmethyldoxepin, and Z-desmethyldoxepin) in a test sample are disclosed. The quantification of total doxepins is accomplished in an immunoassay employing antibodies and labeled reagents prepared with doxepin derivatives of the Formula II: ##STR1## wherein Y-Z can be C=CH or N-CH.sub.2, R.sub.1 is a linking group, R.sub.2 can be H or CH.sub.3, and Q can be a detectable moiety or an immunogenic carrier material. The antibody reagent comprises antibodies which are capable of binding to total doxepins and which are produced with one or more immunogens prepared from the doxepin derivative of Formula II, and the labeled reagent is also prepared from the doxepin derivative of Formula II.

REFERENCES:
patent: 4223013 (1980-09-01), Hu et al.
patent: 4420568 (1983-12-01), Wang et al.
patent: 4629691 (1986-12-01), Collins et al.
patent: 5256409 (1993-10-01), Blincko
M. T. Rosseel, et al, "Quantitative GLC Determination of CIS- and Trans-Isomers of Doxepin and Desmethyldoxepin", J. Pharmaceutical Sciences, vol. 67, pp. 802-805 (1988).
R. Virtanen, et al, "Radioimmunoassay For Doxepin and Desmethyldoxepin", Acta pharmacol., et toxicol., vol. 47, pp. 274-278 (1980).
K. K. Midha, et al, "Radioimmunoassay For Total Doxepin And N-Desmethyl Doxepin In Plasma", Communications in Psychopharmacology, vol., 4, pp. 11-15 (1980).
Y. H. Park, et al, "Quantitative Determination of Doxepin and Nordoxepin in Urine By High-Performance Liquid Chromatography", Journal of Chromatography, vol., 375, pp. 202-206 (1986).
M. N. Al-Bassam, et al, "Nortriptyline Enzyme-Immunoassay" Proc. Int. Symp., Walter de Gruyter & Co., Berlin/New York (Ed.), pp. 375-386 (1978).
I. Lavastre, et al, "The Synthesis Of Metallocene-Labelled Drugs For Biological Assays", Applied Organometallic Chemistry, vol., 4, pp. 9-17 (1990).

Affiliated with

Also associated with

Rating

Reagents and methods for the quantification of total doxepins in does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Reagents and methods for the quantification of total doxepins in, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagents and methods for the quantification of total doxepins in will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-196793