Reagents and methods for the quantification of imipramine or des

Details Reagents and methods for the quantification of imipramine or des Reagents and methods for the quantification of imipramine or des

1994-08-05

1996-11-26

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

435 793, 435188, 436536, 436544, 436546, 5303898, 530404, 530807, 540592, G01N 33542, C12N 996, C07K 1644, C07D22318

Patent

active

055785000

ABSTRACT:
Immunoassay methods and reagents for the specific quantification of imipramine or desipramine in a test sample are disclosed. The measurement of imipramine or desipramine is accomplished in a specific immunoassay employing antibodies prepared with imipramine or desipramine derivatives of the Formula III: ##STR1## wherein P is an immunogenic carrier material, X is two heteroatoms, Y is a linking group comprising from 1 to 6 carbon atoms and P is an immunogenic carrier material, and wherein for imipramine, R is CH.sub.3, and for desipramine, R is H.
The present invention also describes the synthesis of unique labeled reagents of the structure of the Formula IV: ##STR2## wherein Z is a linking group comprising 1 to 4 carbon atoms and 0 to 2 heteroatoms and Q is a detectable moiety, preferably fluorescein or a fluorescein derivative, and wherein for imipramine, R.sub.1 is CH.sub.3, and for desipramine, R.sub.1 is H.

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