Reagents and methods for the quantification of imipramine or des

Details Reagents and methods for the quantification of imipramine or des Reagents and methods for the quantification of imipramine or des

1992-07-24

1994-08-23

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

435 793, 436544, 436546, 436815, G01N 33542, G01N 33533, G01N 33535

Patent

active

053407504

ABSTRACT:
Immunoassay methods and reagents for the specific quantification of imipramine or desipramine in a test sample are disclosed. The measurement of imipramine or desipramine is accomplished in a specific immunoassay employing antibodies prepared with imipramine or desipramine derivatives of the Formula III: ##STR1## wherein P is an immunogenic carrier material, X is two heteroatoms, Y is a linking group comprising from 1 to 6 carbon atoms and P is an immunogenic carrier material, and wherein for imipramine, R is CH.sub.3, and for desipramine, R is H.
The present invention also describes the synthesis of unique labeled reagents of the structure of the Formula IV: ##STR2## wherein Z is a linking group comprising 1 to 4 carbon atoms and 0 to 2 heteroatoms and Q is a detectable moiety, preferably fluorescein or a fluorescein derivative, and wherein for imipramine, R.sub.1 is CH.sub.3, and for desipramine, R.sub.1 is H.

REFERENCES:
patent: 4223013 (1980-09-01), Hu et al.
patent: 4275160 (1981-06-01), Singh et al.
patent: 4420568 (1983-12-01), Wang et al.
patent: 4495281 (1985-01-01), Buckler et al.
patent: 4551275 (1985-11-01), Pirio et al.
patent: 4629691 (1986-12-01), Collins et al.
A. Nagy, et al, "Quantitative Determination of Imipramine and Desipramine in Human Blood Plasma by Direct Densitometry of Thin-Layer Chromatograms", J. Pharm. Pharmac., vol. 25, pp. 509-603 (1973).
T. B. Cooper, et al, "A Sensitive GLC Method for the Determination of Imipramine and Desmethylimipramine Using a Nitrogen Detector", Psychopharmacology Communications, vol. 1(4), pp. 445-454 (1975).
J. W. Hubbard, et al, "Radioimmunoassay for Psychotropic Drugs II: Synthesis and Properties of Haptens for Tricyclic Antidepressants", Journal of Pharmaceutical Sciences, vol., 67(11) pp. 1571-1578 (1978).
K. K. Midha, et al, "Monitoring of Therapeutic Concentrations of Psychotropic Drugs in Plasma by Radioimmunoassays", Journal of Analytical Toxicology, vol. 2, pp. 185-192 (1978).
R. Virtanen, "Radioimmunoassay for Tricyclic Anti-Depressants", Scand. J. Clin. Lab. Invest., vol., 40, pp. 191-197 (1980).
P. K. Sonsalla, et al, "An Evaluation of the Emit.RTM. St Assay for the Direction of Tricyclic Antidepressant Drugs in Plasma or Serum", Clinical Toxicology, vol. 22(1), pp. 63-76 (1984).
S. Pankey, et al, "Quantitative Homogeneous Enzyme Immunoassays for Amitriptyline, Nortriptyline, Imipramine, and Desipramine", Clin. Chem., vol. 32(5), pp. 768-772 (1986).
Syva, "Practical Considerations for the Evaluation of the Emit.RTM. Tricyclic Antidepressant Assays", Emit.RTM. Quantitative Tricyclic Antidepressant Assays, pp. 1-13 (1986).
H. Denis, et al, "Enzyme-Linked Immumosorbent Assay for Amitriptyline and Other Antidepressants Using a Monoclonal Antibody", Clinica Chimica Acta, vol., 159, pp. 257-267 (1986).
A. M. O'Callaghan, et al, "Antisera Raised Against the Drug Imipramine", Journal of Neurochemistry, vol., 49(4), pp. 1091-1095 (1987).
M. Bowles, et al, "Large Scale Production and Purification of Paraquat and Desipramine Monoclonal Antibodies and Their Fab Fragments", Int. J. Immunopharmac., vol., 10(5), pp. 537-545 (1988).
R. Cameron Dorey, et al, "Results Compared for Tricyclic Antidepressants as Assayed by Liquid Chromatography and Enzyme Immunoassay", Clin., Chem., vol., 34(11), pp. 2348-2351 (1988).
D. J. Winzor, et al, "Adaptation of the Muller Method to Allow Quantitative Characterization of the Affinity and Cross-Reactivity of Antibodies by Competitive Radioimmunoassay" Molecular Immunology, vol., 28(9), pp. 995-1001 (1991).

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