Chemistry: analytical and immunological testing – Involving producing or treating antigen or hapten – Producing labeled antigens
Patent
1994-08-05
1997-03-25
Woodward, Michael P.
Chemistry: analytical and immunological testing
Involving producing or treating antigen or hapten
Producing labeled antigens
549223, 540592, 436816, 436547, 436822, 436823, 530363, 5303889, 5303898, 530404, 530405, 530408, 530409, G01N 33533, G01N 3353, G01N 33566, C07K 1700
Patent
active
056144199
ABSTRACT:
Immunoassay methods and reagents for the specific quantification of amitriptyline or nortriptyline in a test sample are disclosed employing antibodies prepared with amitriptyline or nortriptyline derivatives of the Formula III: ##STR1## wherein for amitriptyline, R is CH.sub.3, and for nortriptyline, R is H. The present invention also describes the synthesis of unique fluorescein tracers of the structure of Formula IV and Formula V: ##STR2## wherein for a specific amitriptyline immunoassay, W.sub.1 is a heteroatom linked to the aromatic ring at the 2 or 3 position, and for a specific nortriptyline immunoassay, W.sub.2 is two heteroatoms linked together and attached to the aromatic ring at the 2 or 3 position, and wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative.
REFERENCES:
patent: 4160818 (1979-07-01), Smith et al.
patent: 4223013 (1980-09-01), Hu et al.
patent: 4275160 (1981-06-01), Singh et al.
patent: 4307245 (1981-12-01), Hu et al.
patent: 4420568 (1983-12-01), Wang et al.
patent: 4495281 (1985-01-01), Buckler et al.
patent: 4551275 (1985-11-01), Pirio et al.
patent: 4585862 (1986-04-01), Wang et al.
patent: 4629691 (1986-12-01), Collins et al.
patent: 4668640 (1987-05-01), Wang et al.
patent: 4795822 (1989-01-01), Hu et al.
March, J., "Advanced Organic Chemistry", Fourth Ed., John Wiley, New York, pp. 498-499. 1992.
Aldrich Catalog Handbook of Fine Chemicals, Aldrich Chem. Co., Milwaukee, p. 747. 1988-89.
David L. Black, et al., "Enzyme Immunoassay Method for Comprehensive Drug Screening in Micro-Samples of Urine", Clinical Chemistry, vol. 33, No. 3 (1987), pp. 367-371.
Gerard M. Meenan, et al., "Urinary Tricyclic Antidepressant Screening: Comparison of Results Obtained with Abbott FPIA Reagents and Syva EIA Reagents", Journal of Analytical Toxicology, vol. 14, No. 5 (1990), pp. 273-276.
A. Fazio, et al., "Evaluation of Tricyclic Antidepressant Plasma Levels by an Automated Enzyme Immunoassay (EMIT) in Comparison to a High-Performance Liquid Chromatographic Method", Therapeutic Drug Monitoring, vol. 10, No. 3 (1988), pp. 333-339.
S.Pankey, et al, "Quantitative Homogeneous Enzyme Immunoassays for Amitriptyline, Nortriptyline, Imipramine, and Desipramine", Clin. Chem., vol., 32(5), pp. 768-772 (1986).
H. Denis, et al, "Enzyme-Linked Immunosorbent Assay for Amitriptyline and Other Antidepressants Using a Monoclonal Antibody", Clinica Chimica Acta, vol., 159 pp. 257-267 (1986).
M. Bowles, et al, "Large Scale Production and Purification of Paraquat and Desipramine Monoclonal Antibodies and Their FAB Fragments", Int. J. Immunopharmac., vol., 10,(5), pp. 537-545 (1988).
R. Cameron Dorey, et al, "Results Compared for Tricyclic Antidepressants as Assayed by Liquid Chromatography and Enzyme Immunoassay", Clin. Chem., vol., 34(11), pp. 2348-2351 (1988).
I. Lavastre, et al, "The Synthesis of Metallocene-Labelled Drugs for Biological Assays", Applied Organometallic Chemistry, vol., 4, pp. 9-17 (1990).
D.J. Windzor, et al, "Adaptation of the Muller Method to Allow Quantitative Characterization of the Affinity and Cross-Reactivity of Antibodies by Competitive Radioimmunoassay", Molecular Immunology, vol., 28(9), pp. 995-1001 (1991).
SYVA, "Practical Considerations for the Evaluation of the Emit.RTM. Tricyclic Antidepressant Assays", Emit.RTM. Quantitative Tricyclic Antidepressant Assays, pp. 1-13 (1986).
P.K. Sonsalla, et al, "An Evaluation of the Emit.RTM.ST Assay for the Detection of Tricyclic Antidepressant Drugs in Plasma or Serum", Clinical Toxicology, vol., 22(1), pp. 63-76 (1984).
K.K. Midha, et al, "Monitoring of Therapeutic Concentrations of Psychotrophic Drugs in Plasma by Radioimmunoassays", Journal of Analytical Toxicology, vol., 2, pp. 185-192 (Sep./Oct. 1978).
A. Nagy, et al, "Quantitative Determination of Imipramine and Desipramine in Human Blood Plasma by Direct Densitometry of Thin-Layer Chromatograms", J. Pharmac., vol., 25, pp. 599-603 (1973).
R. Lucek, et al, "Specific Radioimmunoassay for Amitriptyline and Nortriptyline in Plasma", Research Communications in Chemical Pathology and Pharmacology, vol., 18(1), pp. 125-136 (Sep. 1977).
M.N. Al-Bassam, et al, "Double-Antibody Enzyme Immunoassay for Nortriptyline", Clinical Chemistry, vol., 24(9), pp. 1590-1594 (1978).
G.P. Mould, et al, "Radioimmunoassay of Amitriptyline and Nortriptyline in Body Fluids", Annals of Clinical Biochemistry, vol., 15 pp. 221-225 (1978).
J.W. Hubbard, et al, "Radioimmunoassay for Psychotropic Drugs II; Synthesis and Properties of Haptens for Tricyclic Antidepressants", Journal of Pharmaceutical Sciences, vol., 67 (11), pp. 1571-1578 (1978).
W.A. Garland, et al, "A Method for the Determination of Amitriptyline and Its Metabolites Nortriptyline, 10-Hydroxyamitriptyline, and 10-Hydroxynortriptyline in Human Plasma Using Stable Isotope Dilution and Gas Chromatography-Chemical Ionization Mass Spectrometry (GC-CIMS)", Clin. Pharmacol. Ther., vol., 25(6), pp. 844-856 (1979).
R. Virtanen, et al, "Radioimmunoassay for Tricyclic Antidepressants", Scand. J. clin. Lab. Invest., vol. 40, pp. 191-197 (1980).
J.F. Sayegh, "A Simplified Radioimmunoassay of Plasma Nortriptyline in Depressed Patients Compared With High-Pressure Liquid Chromatography and Gas-Liquid Chromatography", Neurochemical Research, vol., 11(2), pp. 193-206 (1986).
Adamczyk Maciej
Fishpaugh Jeffrey R.
Hartter Daryl E.
Johnson Donald
Abbott Laboratories
Auer Henry E.
Levis John F.
Woodward Michael P.
LandOfFree
Reagents and methods for the quantification of amitriptyline or does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Reagents and methods for the quantification of amitriptyline or , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagents and methods for the quantification of amitriptyline or will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2202723