Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Signal modification or steric inhibition
Patent
1992-07-24
1995-04-18
Ceperley, Mary E.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Signal modification or steric inhibition
435 793, 436544, 436546, 436815, G01N 33542, G01N 33533, G01N 33535
Patent
active
054078350
ABSTRACT:
Immunoassay methods and reagents for the specific quantification of amitriptyline or nortriptyline in a test sample are disclosed employing antibodies prepared with amitriptyline or nortriptyline derivatives of the Formula III: ##STR1## wherein for amitriptyline, R is CH.sub.3, and for nortriptyline, R is H. The present invention also describes the synthesis of unique fluorescein tracers of the structure of Formula IV and Formula V: ##STR2## wherein for a specific amitriptyline immunoassay, W.sub.1 is a heteroatom linked to the aromatic ring at the 2 or 3 position, and for a specific nortriptyline immunoassay, W.sub.2 is two heteroatoms linked together and attached to the aromatic ring at the 2 or 3 position, and wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative.
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Adamczyk Maciej
Fishpaugh Jeffrey R.
Hartter Daryl E.
Johnson Donald
Abbott Laboratories
Ceperley Mary E.
Collins Daniel W.
Steele Gregory W.
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