Reagents and methods for the determination of glycohydrolytic en

Details Reagents and methods for the determination of glycohydrolytic en Reagents and methods for the determination of glycohydrolytic en

1992-01-29

1993-12-21

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 4, 435 74, 435 77, 436800, 436808, C07H 1524, C12Q 100

Patent

active

052722600

ABSTRACT:
Novel pyrene-trisulfonate derivatives and the use thereof in a method for the determination of glycohydrolytic enzyme activity are provided. The method comprises the steps of (a) forming a test solution comprising a test sample containing the glycohydrolytic enzyme and a pyrene-trisulfonate derivative of the present invention, wherein the derivative is hydrolyzed by the glycohydrolytic enzyme to result in the formation of free 8-hydroxy-1,3,6-pyrene trisulfonate as a function of, and which can be correlated to, the amount of the glycohydrolytic enzyme present in the test sample, and (b) measuring and correlating either the intensity of fluorescence, or the optical density, of the test solution to the presence or amount of the glycohydrolytic enzyme in the test sample. A preferred pyrene-trisulfonate derivative is pyrene-1,3,6-trisulfonic acid) 8-.beta. -D-glucuronide for the determination of .beta.-D-glucuronidase for the diagnosis of periodontal disease.

REFERENCES:
patent: 4830961 (1989-05-01), Petty
patent: 4912208 (1990-03-01), Fiechtner et al.
patent: 5102808 (1992-04-01), Petty

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