Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1998-05-20
2000-11-28
Ceperley, Mary E.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
435 6, 435 75, 436500, 436531, 436532, 436534, 436544, 436815, 436817, 5483037, G01N 33545, G01N 33532, C07D49504
Patent
active
061534420
ABSTRACT:
The present invention relates to compounds that are bis-biotins. These compounds comprise two biotinyl radicals connected by a chain of atoms, usually at least 16 atoms in length. The bis-biotin is conjugated to a member of a specific binding pair ("sbp member") wherein the chain is not part of the sbp member. Also disclosed are compositions comprising a complex of avidin and a bis-biotin as described above. The compounds and compositions of the invention find use in an assay for an analyte wherein there is employed a reagent system comprising an avidin reagent and a biotin reagent. The improvement of the present invention comprises using as the biotin reagent a bis-biotin as described above. Also disclosed are kits comprising the present bis-biotins and methods of preparing a bis-biotinylated conjugate of a member of a specific binding pair ("sbp member") for use in a specific binding assay.
REFERENCES:
patent: Re29169 (1977-04-01), Schuurs et al.
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3996345 (1976-12-01), Ullman et al.
patent: 4098876 (1978-07-01), Piasio et al.
patent: 4233402 (1980-11-01), Maggio et al.
patent: 4244940 (1981-01-01), Jeong et al.
patent: 4264766 (1981-04-01), Fischer
patent: 4275149 (1981-06-01), Litman et al.
patent: 4298685 (1981-11-01), Parikh et al.
patent: 4318980 (1982-03-01), Boguslaski et al.
patent: 4474878 (1984-10-01), Halbert et al.
patent: 4486530 (1984-12-01), David et al.
patent: 4535057 (1985-08-01), Dreesman et al.
patent: 4707440 (1987-11-01), Stavrianopoulos
patent: 4857453 (1989-08-01), Ullman et al.
patent: 4868104 (1989-09-01), Kurn et al.
patent: 4959303 (1990-09-01), Milburn et al.
patent: 5089390 (1992-02-01), Davalian et al.
patent: 5185243 (1993-02-01), Ullman et al.
patent: 5219764 (1993-06-01), Huber et al.
patent: 5340716 (1994-08-01), Ullman et al.
patent: 5514559 (1996-05-01), Markert-Hahn et al.
Wilbur, et al.; Journal of Labelled Compounds and Radiopharmaceuticals; Investigation of Biotin Dimers and Trimers for Increasing the Quantity of Radioactivity on Cancer Cells in Tumor Pretargeting. in vitro Demonstration of Streptavidin Cross-Linking; 40:335-337; 1997.
Luppa, et al.; Bioconjugate Chem; Synthesis of 3-Hydroxyestra-1,3,5(10)-trien-17-one and 33,17.beta.-Dihydroxyestra-1,3,5(10-triene 6.alpha.-N-(.epsilon.-Biotinyl)caproamide, Tracer Substances for Developing Immunoassays for Estrone and Estradiol; 5/2:167-171; Mar. 1, 1994.
Mares, et al.; Journal of Immunological Methods; Synthesis of a Novel Biotin-Estradiol Conjugate and its Use for the Development of a Direct, Broad Range Enzyme Immunoassay for Plasma Estradiol; 183/2:211-219; Jun. 28, 1995.
Masanori, Oka; Patent Abstracts of Japan; Labeled Primer in Non-Ri Sequencing; vol. 018, No. 371; Jul, 13, 1994 (Abstract of Japanese Patent 061,005/86, Toyobo Co Ltd).
Morgan, et al; Mol Cryst Liq Cryst; Self-Assembly of Streptavidin/Bisbiotin Monolayers and Multilayers; 235:121-126; 1993.
Margel, S.; Affinity Separation with Polyaldehyde Microsphere Beads; J. Chromatography; 462:177-189; 1989.
Welsh, K. I.; Antibody production made easier; Nature; 266:495; Apr. 7, 1977.
Wade, N.; Hybridomas: A Potent New Biotechnology; Science; 208:692-693; 1980.
Galfre, et al.; Preparation of Monoclonal Antibodies: Strategies and Procedures; Methods In Enzymology; 73 (Part B):3-46; 1981.
Green, N. Michael; Avidin and Streptavidin; Methods in Enzymology; 184 Chp. #5:51-67; 1990.
Akiyama et al; Biotin Derivatives of Endothelin: Utilization for Affinity Purification of Endothelin Receptor; Protein Expression and Purification; 3:427-433; 1992.
Basak et al; Biotinylation of an Enkephalin-Containing Heptapeptide via Various Spacer Arms. Synthesis, Comparative Binding Studies toward Avidin and Application as Substrates in Enzymatic Reactions; Bioconjugate Chem.; 5:301-305; 1994.
Morgan et al.; Polymerization of Avidin and Streptavidin with Aromatic Bisbiotin Ligands; J of Polymer Science, Part A Polymer Chem; 32:1331-1340; 1994.
Pierlot et al; Solid Phase Synthesis of 5' Non Radioactive Multiple Labelled Oligodesoxyribonucleotides; Journal of Med Chem Letters: 2:267-270; 1992.
Weber et al.; Structural Origins of High-Affinity Biotin Binding to Streptavidin; Science; 243:85-88; Jan. 6, 1989.
Nutikka et al; Synthesis and Structural Characterization of a Cardioactive Biotinylated Digoxin Analogue; Clin Biochem; 24:469-473; 1991.
Green et al.; The Use of Bifunctional Biotinyl Compounds to Determine the Arrangement of Subunits in Avidin; Biochem. J. (GB); 125:781-791; 1971.
Livnah et al; Three-Dimensional Structures of Avidin and the Avidin-Biotin Complex; Proc Natl Acad Sci.; 90:5076-5080; Jun. 1993.
Cuatrecases, P.; Protein Purification by Affinity Chromatography; J. Biol. Chem. 245:3059-3065, 1970.
Yallow, et al. Immunoassay of Endogenous Plasma Insulin in Man; J. Clin Invest; 39:1157-1175, 1960.
Kohler & Milstein; Continuous cultures of fused cells secreting antibody of predefined specificity; Nature; 256:495-497, 1975.
Wilbur et al; Biotin Reagents for Antibody Pretargeting. 2. Synthesis and in Vitro Evaluation of Biotin Dimers and Trimers for Cross-Linking of Streptavidin; Bioconjugate Chem.; 8:819-832; 1997.
Cook et al; Synthesis and hybridization of a series of biotinylated oligonucleotides; Nucleic Acids Research; 16 #9:4077-4095; 1988.
Green, N.M.; Avidin; Adv. Protein, Chem.; 29;85-133; 1975.
Davalian Dariush
Ishkanian Jacqueline Sadakan
Pirio Marcel Rene
Ullman Edwin F.
Ceperley Mary E.
Dade Behring Inc.
Gattari Patrick G.
LandOfFree
Reagents and methods for specific binding assays does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Reagents and methods for specific binding assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagents and methods for specific binding assays will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1724214