Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1995-02-02
1997-12-02
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4352401, 436 8, 436 86, 536 243, 536 2433, C12N 910
Patent
active
056935170
ABSTRACT:
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
REFERENCES:
patent: 4133717 (1979-01-01), Johnson et al.
patent: 4563406 (1986-01-01), Ohbayashi et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5310893 (1994-05-01), Erlich et al.
patent: 5422242 (1995-06-01), Young
Kallen et al., 1962, "The Occurrence of a New Pyrimidine Base Replacing Thymine in a Bacteriophage DNA: 5-Hydroxymethyl Uracil" J. Mol. Biol. 5:248-250.
Good et al., 1966, "Hydrogen Ion Buffers for Biological Research" Biochemistry 5(2):467-477.
Karkas et al., 1972, "Action of DNA Polymerase I of Escherichia coli With DNA-RNA Hybrids as Templates" Proc. Natl. Acad. Sci. USA 69(2):398-402.
Karkas, 1973, "Reverse Transcription by Escherichia coli DNA Polymerase I" Proc. Natl. Acad. Sci USA 70(12):3834-3838.
Leob et al., 1973, "Copy of Natural RNAs with E. coli DNA Polymerase I" Nature New Biology 242:66-69.
Gulati et al., 1974, "Conditions for Using DNA Polymerase I as an RNA-Dependent Polymerase" Proc. Natl. Acad. Sci. USA 71(4):1035-1039.
Temin and Mizutani, Chapter 7, The Enzymes, "RNA Tumor Virus DNA Polymerases" Paul Boyer, Academic Press, Inc. London, 1974 ed.
Travaglini and Leob, "Ribonucleic Acid Dependent Deoxyribonucleic Acid Synthesis by Escherichia coli Deoxyribonucleic Acid Polymerase I. Characterization of the Polymerization Reaction" Biochemistry 13(15):3010-3017.
Perrin and Dempsey, Buffers for pH and Metal Ion Control, Chapter 7, Entitled "Metal Ion Buffers" pp. 94-108 New York, Chapman and Hall, 1974.
Chien et al., 1976, "Deoxyribonucleic Acid Polymerase From the Extreme Thermophile Thermus aquaticus" J. Bacteriology 127(3):1550-1557.
Houdebline, 1976, "Synthesis of DNA Complementary to the mRNAs for Milk Proteins by E. coli DNA Polymerase I" Nucleic Acids Res. 3(3):615-630.
Mizutani and Temin, 1976, "Incorporation of Noncomplementary Nucleotides at High Frequencies by Ribodeoxyvirus DNA Polymerases and Escherichia coli DNA Polymerase I" Biochemistry 15:1510-1516.
Travaglini et al., 1976, "Template Recognition and Chain Elongation in DNA Synthesis in Vitro" J. Mol. Biol. 106:605-621.
Kaledin et al., 1980, "Isolation and Properties of DNA Polymerase From Extremely Thermophilic Bacterium Thermus aquaticus YT1" Biokhimiya 45(4):644-651.
"Metal Ions in Genetic Information Transfer" in Advances in Inorganic Biochemistry vol. 3, pp. 26-29, 32-35, and 42-46, published by Elsevier North Holland Inc. New York, N.Y. Eds. Eichorn, Gunther, Louis and Marzill, Luigi, 1981.
Ruttimann et al., 1985, "DNA Polymerase From the Extremely Thermophilic Bacterium Thermus thermophilius HB-8" Eur. J. Biochem. 149:41-46.
Jones and Foulkes, 1989, "Reverse Transcription of mRNA by Thermus aquaticus DNA Polymerase" Nuc. Acids Res. 17(20):8387-8388.
Lawyer et al., 1989, "Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene From Thermus aquaticus" J. Biol. Chem. 264(11):6427-6437.
Shimomaye and Salvato, 1989, "Use of Avian Myeloblastosis Virus Revrse Transcriptase at High Temperature for Sequence Analysis of Highly Structured RNA" Gene Anal. Techn. 6:25-28.
Tabor and Richarson, 1989, "Effect of Manganese Ions on the Incorporation of Dideoxynucleotides by Bacteriophage T7 DNA Polymerase and Escherichia coli DNA Polymerase I" Proc. natl. Acad. Sci. USA 86:4076-4080.
Mocharla et al., 1990, "Coupled Reverse Transcription-Polymerase Chain Reaction (RT-PCR) as a Sensitive and Rapid Method for Isozyme Genotyping" Gene 93:271-275.
Shaffer et al., 1990, "Amplification, Detection, and Automated Sequencing of Gibbon Interleukin-2 mRNA by Thermus aquaticus DNA Polymerase Reverse Transcription and Polymerase Chain Reaction" Analytical Biochemistry 190:292-296.
Tse and Forget, 1990, "Reverse Transcription and Direct Amplification of Cellular RNA Transcripts by Taq Polymerase" Gene 88:293-296.
Kawasaki, 1989, "Amplification of RNA Sequences via Complementary DNA (cDNA)" Amplifications 3:4-6.
Krishnamoorthy and Nakon, 1991, "Free Metal Ion Depletetion by Good's Buffers", IV, Bicine 1:1 and 2:1 Complexes With Mg(II), Ca(II), Mn(II), Co(II), Ni(II), Cu(II), and Zn(II) J. Coord. Chem. 23:233-243.
Levy and Teebor, 1991, "Site Directed Substitution of 5-Hydroxymethyluracil for Thymine in Replicating oX-174am3 DNA via Synthesis of 5-Hydroxymethyl2'-Deoxyuridine-5'-Triphospate" Nuc. Acids Res. 19(12):3337-3343.
Lou et al., 1991, "Renin Gene Expression in Various Tissues Determined by Single-Step Polymerase Chain Reaction" Clin. Exp. Pharm. Physiology 18:357-362.
Myers and Gelfand, 1991, "Reverse Transcription and DNA Amplification by a Thermus thermophilus DNA Polymerase" Biochemistry 30:7661-7666.
Ponce and Micol. 1992, "PCR Amplification of Long DNA Fragments" Nuc. Acids Res. 20(3):623.
Brown, Basic Principles in Nucleic Acid Chemistry 2:41-45, Academic Press, New York, N.Y., 1974.
Gelfand David H.
Myers Thomas W.
Sigua Christopher L.
Freed Rachel Heather
Hutzell Paula K.
Johnston George W.
Petry Douglas A.
Roche Molecular Systems Inc.
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