Reagents and method for detecting polychlorinated biphenyls

Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase

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435 79, 435 792, 435 793, 435962, 435968, 436546, 436822, 436825, 549223, G01N 33536

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051457903

ABSTRACT:
Reagents and an immunoassay for detecting the presence or amount of polychlorinated biphenyls in a test sample. The assay is performed by adding a known concentration of a tracer labeled with a detectable moiety and a known concentration of an analyte-specific antibody to a test sample to form a mixture, incubating the mixture to form labeled tracer-antibody and analyte-antibody complexes, and determining the presence or amount of tracer-antibody complexes formed as a measure of the presence or amount of analyte in the test sample. Reagents provided include tracers, immunogens and an additive compound useful in preventing non-specific binding of the polychlorinated biphenyls to proteins which may be present in the test sample. A kit for performing the assay also is provided.

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