Reagents and kits for determining polymerase activity

Details Reagents and kits for determining polymerase activity Reagents and kits for determining polymerase activity

1995-02-03

1997-06-03

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 15, 435183, 435810, C12Q 168

Patent

active

056353502

ABSTRACT:
Method of determining polymerase activity by incubating the polymerase with a template nucleic acid, one detectable mononucleoside triphosphate and one immobilizable nucleoside triphosphate, binding the immobilizable nucleotides to a solid phase and detecting the bound delectable nucleotides and tests based thereon.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4851331 (1989-07-01), Vary et al.
Advani et al. J. Clin. Microb. 27(7) 1453-1455 (1989).
Lee et al. J. Clin. Microb. 25(9) 1717-1721 (1987).
Day et al. Biochem. Journal 267: 119-123 (1990).
Arias et al. J. Biol. Chem. 264(6): 3223-3229 (1989).

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