Reagentless, reusable, bioelectronic detectors

Details Reagentless, reusable, bioelectronic detectors Reagentless, reusable, bioelectronic detectors

2011-08-23

2011-08-23

Crow, Robert T. (Department: 1634)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S006120, C435S283100, C435S287100, C536S024300

Reexamination Certificate

active

08003374

ABSTRACT:
A reagentless, reusable bioelectronic DNA, or other oligonucleotide sequence sensor is disclosed. The sensor includes an oligonucleotide (aptamer) probe tagged with a electroactive, redoxable moiety, self-assembled on or near an electrode. This surface-confined oligonucleotide (aptamer) probe structure undergoes hybridization-induced conformational change in the presence of the target which changes the electron-transfer distance between the redoxable moiety and the electrode thereby providing a detectable signal change. In an alternative embodiment, the target can harbor the redoxable moiety.

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