Reagent useful for detection and quantitative determination of l

Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preparation composition

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

436 18, 436904, 252700, G01N 3349

Patent

active

051282658

ABSTRACT:
A reagent for the detection and quantitative determination of leukocytes by measuring the myeloperoxidase (MPO) activity of biological samples, which is sensitive for disclosing even only a few leukocytes, without interferences caused by hemoglobin even in the presence of several erythrocytes, and suitable for photometric readings in the visible spectrum region. The reagent comprises a buffer, a chromogen, a surface-active agent, at least an alkali metal halide, a hydroperoxide compound and optionally a reaction promoter.

REFERENCES:
patent: 3087794 (1963-04-01), Free et al.
patent: 3886045 (1975-05-01), Meiattini
patent: 4295853 (1981-10-01), Kasahara et al.
patent: 4721670 (1988-01-01), Osada et al.
patent: 4755472 (1988-07-01), Ismail et al.
patent: 4851353 (1989-07-01), Miike et al.
Tomankova, H. et al. "The Oxidation of Aminophenazone by Hydrogen Peroxide" Microchemical Journal, vol. 20, 132-153 (1975).
Bos, A. J. et al. "Some Enzymatic Characteristics of Eosinophil Peroxidase from Patients with Eosinophilia and from Healthy Donors." Infection and Immunity, vol. 32, No. 2, 1981, pp. 427-431.
Meiattini, F. et al. "The 4-Hydroxybenzoate/4-Aminophenazone Chromogenic System Used in the Enzymic Determination of Serum Cholesterol." Clinical Chemistry, vol. 24, No. 12, 1978, pp. 2161-2165.
Cowman, R. A. "Evidence for Thiocyanate-Sensitive Peroxidase Activity in Human Saliva." Journal of Clinical Microbiology, vol. 18, No. 5, 1983, pp. 1177-1182.
Matheson, N. R. "Isolation and Properties of Human Neutrophil Myeloperoxidase." Biochemistry, vol. 20, 1981, pp. 325-330.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Reagent useful for detection and quantitative determination of l does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Reagent useful for detection and quantitative determination of l, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent useful for detection and quantitative determination of l will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1829353

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.