Reagent systems, enzymatic assays and compositions therefor

Details Reagent systems, enzymatic assays and compositions therefor Reagent systems, enzymatic assays and compositions therefor

1977-11-09

1980-02-19

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

435 14, 435 17, G01N 3114

Patent

active

041895363

ABSTRACT:
An improved reagent system for chemical determinations and novel compositions therefor provide improved reagent stability, particularly in a dry, storage stable phase. For determinations in which hexokinase and glucose-6-phosphate dehydrogenase are indicator enzymes, glycine and taurine are combined with bovine serum albumin to provide a bulking agent for the indicator enzymes which also acts as a stabilizer. A novel salt, the tris (hydroxymethyl)aminomethane salt of 2 mercaptosuccinic acid, acts as an activator or stabilizer in chemistries utilizing the indicator enzymes, namely determinations of creatine phosphokinase or glucose. The novel salt also acts as a stabilizer in a urea nitrogen determination. Dry reagent components are bulked in triethanolammonium terephthalate. Novel CPK, glucose and serum urea nitrogen determinations are also provided.

REFERENCES:
patent: 3527674 (1970-09-01), Deutsch
patent: 3867258 (1975-02-01), Forgione
patent: 3956069 (1976-05-01), Allain et al.
patent: 4022667 (1977-05-01), Myrick et al.

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