Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1996-10-15
1998-10-06
Smith, Lynette F.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 2, 435721, 436512, 436513, 436518, 436526, 436531, C12Q 170, A01N 102, G01N 3353, G01N 33567
Patent
active
058174583
ABSTRACT:
Fluorometric immunological assay method for detection of HIV-1 infection in which Murine anti-gp120 monoclonal antibodies coupled to paramagnetic microspheres (14) and Fluorescein conjugated anti-gp120 polyclonal antibodies IgG (16) are incubated in a few drops of whole blood diluted in 0.5 cc phosphate buffered saline (10). After incubation for 5 minutes, the HIV-infected peripheral blood lymphocytes (18) will be coated with both the Murine anti-gp120 monoclonal antibodies coupled to paramagnetic microspheres (14) and Fluorescein conjugated anti-gp120 polyclonal antibodies IgG (16) at exposed gp120 antigens (20) binding sites. At the time of measurement said HIV- infected peripheral blood lymphocytes (18) will be pulled against the wall of the measurement vessel by means of a magnetic gradient (26). The cells adhering to the vessel wall are illuminated at 488 nm monochromatic light by a focused light source (28) and the resultant emitted fluorescence is imaged, measured and recorded.
REFERENCES:
patent: 4487830 (1984-12-01), Coates
patent: 4659678 (1987-04-01), Forrest et al.
patent: 4731337 (1988-03-01), Luotola et. al.
patent: 4777145 (1988-10-01), Luoto
patent: 4935147 (1990-06-01), Ullman, V
patent: 5166050 (1992-11-01), Shriver
patent: 5217895 (1993-06-01), Ohno
patent: 5238810 (1993-08-01), Fujiwara
patent: 5245015 (1993-09-01), Fung
patent: 5279936 (1994-01-01), Vorpahl
patent: 5411863 (1995-05-01), Miltenyl
patent: 5541072 (1996-07-01), Wang et al.
patent: 5543289 (1996-08-01), Miltenyi
Burke et al., "Measured of the Fals Positive Rate in a Screening Program for Human Immunodeficiency Virus Infections", New England J. Med., 319:961-964 (1988).
Busch et al., "Consistent Sequential Detection of RNA, Antigen and Antibody in Early HIV Infection: Assessment of the Window Period", XI International AIDS Conference (1996).
Chanh et al., "Human immundeficiency virus gp 120 glycoprotein detected by a monoclonal antibody to synthetic peptide", Eur. J. Immunol., 16:1465 (1986).
Centers for Disease Control, "Update" Serologic Testing for Antibody to Human Immunodeficiency Virus, MMWR, 36:833-840 (1988).
Cohen et al., "Transmission of Retroviruses by Transfusion of Screened Blood in Patient Undergoing Cardiac Surgery", New England J. Med., 320:1172-1176, (1989).
Dalgeleish et al., Science, 312:763-767 (1984).
Dutton et al., "Iron-dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution immunelectron microscopy", Natl. Acad. Sci, 76:3392-3396 (1979).
Gallo, "The First Human Retrovirus", Scientific American, 255:88-98 (1986).
Gallo, "The AIDS Virus", Scientific American, 256:47-56 (1987).
Gostling et al., "Monoclonal Antibodies to gp110 and gp41 of Human Immunodeficiency Virus", J. Clin. Microbiol., 25:845 (1987).
Ithakissios et al., "Use of Protein Containing Magnetic Microparticles in Radioassays", Clin. Chem., 23:2072-2079 (1977).
Birkmeyer, et al.: Application of Novel Chromium Dioxide Particle . . . : Clin. Chem.: v. 33/9: pp. 1543-1547, 1987.
Luk et al.: Rapid and sensitive detection of salmonella . . . : J. Immun. Meth.: 137: pp. 1-8, 1991.
Levy, et al.: Isolation of lymphatic retrovirus . . . : Science: vol. 225: pp. 840-842, 1984.
Gallo, et al.: Frequent detection and isolation of cytopathic . . . : Science: vol. 224: pp. 500-503, 1984.
Levy, et al.: Isolation of lymphocytopathic retroviruses . . . : Science: vol. 225: pp. 840-842, 1984.
Gallo, et al.: Frequent detection and isolation of cytopathic . . . :Science: vol. 224: pp. 500-503, 1984.
Birkmeyer, et al.: Application of NOvel Chromium Dioxide Particles . . . : Clin. Chem.: v. 33/9: pp. 1543-1547, 1987.
Luk, et al.: Rapid and sensitive detection of salmonella . . . : J. Immun. Meth.: 137: pp. 1-8, 1991.
Dalgleish et al., "The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus", Nature, vol. 312 (20/27), pp. 763767, Dec. 1984.
Hallowitz Robert A.
King Chester F.
Nelson Brett
Smith Lynette F.
The Avriel Group, AMCAS Division Inc.
LandOfFree
Reagent system for detecting HIV-infected peripheral blood lymph does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Reagent system for detecting HIV-infected peripheral blood lymph, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent system for detecting HIV-infected peripheral blood lymph will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-75480