Reagent system and method for the differentiation and identifica

Chemistry: analytical and immunological testing – Biological cellular material tested

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436 8, 436 10, 436 17, 436 18, 436164, 436166, G01N 3348, G01N 3100

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active

057337845

ABSTRACT:
Whole blood is mixed with a reticulocyte reagent system that has a reticulocyte staining reagent and a diluent reagent, used in combination. This mixture is incubated at room temperature for between about 15 minutes to about 4 hours. The incubated mixture is then analyzed and the light scattering properties of the cells are detected, collected, differentiated and quantitized. Data gathering includes, at least, 10 and 90 degree light scatter detection.

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Reticulocyte Counting by Flow Cytometry Proposed Guideline NCCLS Document H44-P vol. 13 No. 18 Nov. 1993.

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