Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1990-11-13
1993-02-23
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
2504612, 435 29, 435 34, 435 39, 436 17, 436 63, 436519, 436536, 436546, G01N 33569
Patent
active
051889358
ABSTRACT:
Specified classes and subclasses of leukocyte blood cells are identified by immunohematology procedures, based on utilization of antigenic determinants on the cell surface, their reactivity with antibodies which fluoresce under known circumstances, and identified by utilization of principles of flow cytometry or morphology.
This invention particularly concerns improvements in the lysing and fixing method used prior to detection and identifying of the cells. In this method, a whole blood sample first is incubated with a reagent including antibodies to the cell subclass to be identified, the antibodies having directly or indirectly made fluorescently responsive to a particular light (e.g. argon ion laser). The red blood cells then are lysed with a reagent containing saponin. Next follows a leukocyte fixing treatment, preferably using a cross-linking dialdehyde composition, such as glyoxal or glutaraldehyde.
Details are given as to time, temperature, concentration, and the use of additives and stains, as well as the use of particular monoclonal antibodies in the preferred procedure.
REFERENCES:
patent: 3446751 (1969-05-01), Weichselbaum
patent: 3710933 (1973-01-01), Fulwyler et al.
patent: 3883247 (1975-05-01), Adams
patent: 3884579 (1975-05-01), Mauthner
patent: 3916205 (1975-10-01), Kleinerman
patent: 4284412 (1981-08-01), Hansen et al.
patent: 4286963 (1981-09-01), Ledis et al.
patent: 4420558 (1983-12-01), De Mey et al.
patent: 4492752 (1985-01-01), Hoffman et al.
patent: 4493821 (1985-01-01), Harrison
patent: 4499052 (1985-02-01), Fulwyler
patent: 4569946 (1986-02-01), LeVeen
patent: 4717655 (1988-01-01), Fulwyler
patent: 4751179 (1988-06-01), Ledis et al.
Hawley, The Condensed Chemical Dictionary, New York, Van Nostrand Reinhold, Tenth edition, 1981, p. 959.
Hoffman et al. Proceedings National Academy Science USA, vol. 77, No. 8, Aug. 1980, pp. 4914-4917.
Salzman et al., Acta Cytologica, vol. 19, No. 4, 1975, pp. 374-377.
The Merck Index, tenth edition (Windholz editor), Merck & Co., Rahway, N.J., (1983), p. 647.
D. Y. Mason et al. Annals N.Y. Academy of Science vol. 420, pp. 127-138 (1983).
R. A. Thomas et al. J. Histochemistry and Cytochemistry, vol. 25, No. 7, pp. 827-835 (1977).
James R. Downing et al, Laboratory Management, May 1984, pp. 29-37.
R. C. Leif et al, Clinical Chemistry 23, 1492-1498 (1977).
Feinberg Robert I.
Ledis Stephen L.
Leif Robert C.
Coulter Electronics Inc.
Hibnick Gerald R.
Hill Jr. Robert J.
Sparks Meredith P.
LandOfFree
Reagent system and method for identification, enumeration and ex does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Reagent system and method for identification, enumeration and ex, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent system and method for identification, enumeration and ex will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2204103