Reagent system and method for assaying peptidase enzymes

Details Reagent system and method for assaying peptidase enzymes Reagent system and method for assaying peptidase enzymes

1978-12-28

1981-04-07

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 26, C12Q 132, C12Q 136

Patent

active

042606810

ABSTRACT:
A reagent system for assaying a biological specimen for a peptidase enzyme comprising a peptide substrate in which the C-terminal amino acid is dehydroalanine, the enzyme lactate dehydrogenase, the reduced coenzyme nicotinamide adenine dinucleotide and a buffer material.

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patent: 3925162 (1975-12-01), Kanno
patent: 4006061 (1977-02-01), Weeks et al.
patent: 4116774 (1978-09-01), Minato et al.
Kojima et al., Clinica Chimica Acta, 93 (1979) 181-187.
Patchornik et al., JACS, 86, 1206 (1964).
Shifrin et al., J. Biol. Chem., 234, 1555 (1959).
Doherty et al., J. Biol. Chem., 147, 617 (1943).
Bergmeyer, Methods of Enzymatic Analysis, Academic Press, New York, 949-954 (1974).

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