Reagent stabilization in enzyme-donor and acceptor assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 18, 435188, 435810, G01N 3353, C12N 996

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active

049562746

ABSTRACT:
A method of stabilizing .beta.-galactosidase peptide fragments against storage degradation for use in a complementation assay which comprises storing the peptide fragment in a storage medium containing an ionic surfactant or a surfactant derived from a sugar residue and adding a cyclodextrin to the storage medium after storing the peptide fragments but before using them in a complementation assay.

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patent: 4378428 (1983-03-01), Farina et al.
patent: 4708929 (1987-11-01), Henderson
Szejtli, J., Industrial Applications of Cyclodextrins in "Inclusion Compounds III", Academic Press, London, (1984), pp. 287-309.
Turro et al., article in Journal of the American Chemical Society (1982) 104:1789-1794.
J. W. Callahan, et al., Chemical Abstracts, vol. 85, No. 1, (1976), Effects of Detergents on the Hydrolysis of Glycolipids by .beta.-Galactosidase, p. 145.
A. Hersey, et al., Chemical Abstracts, vol. 106, No. 3, (1987), Mechanism of Inclusion Compound Formation for Binding of Organic Dyes, Ions, and Surfactants .alpha.-Cylodextrin Studied by Kinetic Methods Based on Competition Experim., p. 18965.

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