Reagent polynucleotide complex with multiple target binding regi

Details Reagent polynucleotide complex with multiple target binding regi Reagent polynucleotide complex with multiple target binding regi

1985-09-19

1988-02-16

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435810, 436501, 935 77, 935 78, 536 26, 536 27, 536 28, C12Q 168, G01N 33566

Patent

active

047255366

ABSTRACT:
Reagent complexes containing a probe polynucleotide with at least two target binding regions. A labeled polynucleotide is bound to at least a portion of each target binding region. In one method, sample nucleic acid displaces labeled polynucleotide from one target binding region; after washing, a reagent polynucleotide displaces labeled polynucleotide from the other target binding region. In a second method, sample nucleic acid strands having two target nucleic acid sequences in proximity (e.g., sequences translocated in certain cancers) displace the labeled polynucleotide from both target binding region.

REFERENCES:
patent: 4563417 (1986-01-01), Albarella et al.

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