Reagent formulations for glucose assay

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195 63, 195 68, 195 99, 1951035C, G01N 3114

Patent

active

039532950

ABSTRACT:
Glucose is assayed in biological specimens using granular, water soluble, substantially anhydrous, storage-stable reagent formulations containing enzymes, a chromogen and/or a buffer and a nitrogen - containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine with propylene oxide and ethylene oxide in the presence of a catalyst. The surfactant contains polyoxypropylene chains having an average molecular weight of between about 750 and about 6,750, and polyoxyethylene chains constituting between about 10 and about 80 weight percent. The surfactant has an advantageous effect on granulation, dissolution and storage stability of the reagent formulations and is effective for solubilizing protein such as enzymes.

REFERENCES:
patent: 3321019 (1968-02-01), Hammer et al.
patent: 3413198 (1968-11-01), Deutsch
patent: 3627688 (1971-12-01), McCarty et al.
patent: 3858854 (1975-01-01), Won et al.

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