Reagent for use in automatic analyzers for distinguisher leukocy

Details Reagent for use in automatic analyzers for distinguisher leukocy Reagent for use in automatic analyzers for distinguisher leukocy

1990-11-19

1993-08-03

Housel, James C.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

436 17, 436 18, 436 63, 424 3, 424 71, G01N 3100

Patent

active

052328570

ABSTRACT:
The present invention relates to a reagent and to a method of using the same for automatically determining at least one leukocyte sub-population from a total blood sample. Using this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.

REFERENCES:
patent: 4286963 (1981-09-01), Ledis et al.
Kass et al (1981) Am. J. Clin. Pathol. 76:810-12.

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