Reagent for use in a sandwich solid-phase enzyme-immunoassay and

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435188, 435810, 436531, 436533, 436817, 436818, G01N 33535, G01N 33545, G01N 3374, C12N 996

Patent

active

046939695

ABSTRACT:
A reagent for use in a "sandwich" enzyme-immunoassay comprising a polymer of a bioactive substance-specific antibody:enzyme conjugate, wherein said antibody is conjugated to said enzyme by means of heterobifunctional cross-linking agent and a "sandwich" enzyme-immunoassay employing the polymer.

REFERENCES:
patent: 4214048 (1980-07-01), Kitagawa
Enzyme-Immunoassay, Maggio (Ed.) (1980), CRC Press, Inc., Fla., pp. 171, 174, 175 and 192.
Schuurs et al., "Clinica Chimica Acta", 81 (1977), pp. 4, 6, 9, 28 and 29.

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