Reagent for the determination of lipase and process for preparin

Details Reagent for the determination of lipase and process for preparin Reagent for the determination of lipase and process for preparin

1980-01-28

1982-08-10

Lovering, Richard D.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

252312, 252314, 252408, C12Q 144

Patent

active

043438970

ABSTRACT:
A reconstitutable dry reagent for the turbidimetric determination of lipase, which reagent forms an emulsion upon adding water and comprises substrate oil, protective colloid, emulsifier and activator; and the process for making said dry reagent comprising preparing an aqueous emulsion of a triglyceride containing bile acid salt, colipase, at least 10% by weight of protective colloid, and at least a part of a preserving agent, lyophilizing the emulsion, mixing the lyophilizate with a buffer substance and urea, and recovering said dry reagent.

REFERENCES:
patent: 3527674 (1970-09-01), Deutsch
patent: 3898130 (1975-08-01), Komatsu
patent: 3917515 (1975-11-01), Goldberg
patent: 4115313 (1978-09-01), Lyon et al.

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