Reagent for the determination of chlorine ion

Details Reagent for the determination of chlorine ion Reagent for the determination of chlorine ion

1991-06-10

1993-07-20

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 18, 435202, 435204, 435183, 435184, 435810, C12Q 140, C12Q 134, C12N 999

Patent

active

052292703

ABSTRACT:
Quantitative assay and reagent for the determination of chlorine ion in body fluid. Body fluid is contacted with a reagent which includes deactivated .alpha.-amylase, a compound capable of chelating calcium ion, a calcium chelate compound, and an .alpha.-amylase activity-measuring substance; the amount of .alpha.-amylase activity is proportional to the amount of chlorine ion present in the body fluid. The assay is suitable for automation.

REFERENCES:
patent: 4472489 (1984-09-01), McCroskey
patent: 4698300 (1987-10-01), Henkel et al.
Dolabdjian, B., et al., Chemical Abstracts vol. 101, No. 5, abstract No. 34892m (1984).
Henkel, E., et al., Chemical Abstracts, vol. 102, No. 13, abstract No. 108927w (1985).
Nat. Bur. Stand. (Nov. 79) U.S. Dept. of Commerce (NTIS) PB80-110117 NBS Spec. Pub. 260-67.
Oesch et al. (1986) Clin. Chem. 32(8) 1448-1459.
Schales et al. (1941) J. Biol. Chem. 148:879-884.
J. Sendroy (1942) J. Biol. Chem., 142:171-173.
Zall et al. (1956) Anal. Chem. 28:1665-1668.
Levitzhe et al, "The Allosteric Activation of Mammalian .delta.-Amylase by Chloride", pp. 171-180, 1974.
Bergmeyer, "Methods of Enzymatic Analysis", 3rd Edition, vol. IV, pp. 157-160, 1984.
Tietz, "Textbook of Clinical Chemistry", 1986, p. 729.

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