Reagent for the determination of .beta.-lactamase

Details Reagent for the determination of .beta.-lactamase Reagent for the determination of .beta.-lactamase

1981-05-20

1983-05-10

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 27, 435 34, 435 39, 435810, 435 25, 435805, C12Q 104, C12Q 106, C12Q 130, C12Q 138

Patent

active

043830323

ABSTRACT:
The present invention provides a process for the determination of .beta.-lactamases by the use of 7-cyanoacetylaminocephalosporanic acid and measurement of the colored material thereby formed, wherein ammonium ions are added to the material to be tested, together with phosphate ions and/or an agent splitting off oxygen.
The present invention also provides a reagent for the determination of .beta.-lactamases based upon 7-cyanoacetylaminocephalosporanic acid, which reagent additionally contains ammonium ions, together with phosphate ions and/or an agent splitting off oxygen.

REFERENCES:
patent: 4225669 (1980-09-01), Melnick et al.
patent: 4234683 (1980-11-01), McMillan
patent: 4311794 (1982-01-01), Melnick et al.
Madler et al., Applied and Environmental Microbiology, 32(4), 575-578 (1976).

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