Reagent for the determination by hemagglutination of antibodies

Details Reagent for the determination by hemagglutination of antibodies Reagent for the determination by hemagglutination of antibodies

1989-10-12

1994-06-07

Bidwell, Carol E.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is a biological cell or cell fragment

436521, 436532, 436808, 435 732, 435975, 435967, G01N 33556, G01N 33569

Patent

active

053189132

ABSTRACT:
The invention relates to a method of preparing a reagent for the determination by hemagglutination of antibodies to bacterial toxins. According to this method erythrocytes are treated with glutaraldehyde and then with the bacterial toxins in the presence of glutaraldehyde without a wash step. The reagent thus obtained is further treated with a reagent for blocking aldehyde groups.

REFERENCES:
patent: 4403037 (1983-09-01), Coates
patent: 4569919 (1986-02-01), Toth et al.
Relyveld et al., "Preparation of vaccines using glutaraldehyde", Chemical Abstracts, vol. 83, Abstract No. 65363d, p. 437 (1975).
Sundick et al., "Agglutination Reactions", Methods in Immunodiagnosis, Rose & Bigazzi, Eds., 1980, pp. 109-127.

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