Reagent for specific determination of fructosamine

Details Reagent for specific determination of fructosamine Reagent for specific determination of fructosamine

1992-07-09

1994-02-22

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 10, 435 14, 435 25, 435 27, 435 28, 435 87, 530322, 530395, 530375, 530345, 530402, C12Q 100, C12P 1938, A61K 3702, A61K 3578

Patent

active

052886069

ABSTRACT:
The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto.
The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.

REFERENCES:
patent: 4166763 (1979-09-01), Esders et al.
patent: 4168205 (1979-09-01), Danninger et al.
patent: 4455371 (1984-06-01), Richardson et al.
patent: 4610963 (1986-09-01), Matsui et al.
patent: 4642295 (1987-02-01), Baker
patent: 4956301 (1990-09-01), Ismail et al.
patent: 5002893 (1991-03-01), Rosenthal
patent: 5055388 (1991-10-01), Vogt et al.

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