Reagent for simultaneous determination of hemoglobin and leukocy

Details Reagent for simultaneous determination of hemoglobin and leukocy Reagent for simultaneous determination of hemoglobin and leukocy

1990-10-10

1993-10-05

Housel, James C.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

436 8, 436 17, 436 18, 436 63, 436 66, G01N 3348

Patent

active

052504379

ABSTRACT:
A reagent for measuring the hemoglobin concentration and counting the number of leukocytes in a blood sample, which contains at least one of a quaternary ammonium salt or a pyridinium salt, having a concentration capable of hemolyzing erythrocytes in the blood and denaturing hemoglobin and at least one of cationic, nonionic or amphoteric surfactants or an oxidant capable of oxidizing heme in hemoglobin, the reagent being capable of dividing leukocytes into two or three fractions, for example, a fraction of lymphocytes, a fraction of monocytes, eosinophils and basophils, and a fraction of neutrophils.

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Clinical Biochemistry, vol. 15, No. 1, 1982, pp. 83-88, Pergamon Press, Ottawa, CA; I. Oshiro et al.: "New Method for Hemoglobin Determination By Using Sodium Lauryl Sulfate (SLS)".

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