Reagent for reticulocyte counting by flow cytometry

Details Reagent for reticulocyte counting by flow cytometry Reagent for reticulocyte counting by flow cytometry

1987-11-04

1991-01-01

Warden, Robert J.

Chemistry: analytical and immunological testing

Biological cellular material tested

435 2, 435 4, 435 35, 436 56, 436172, 436519, 436520, 436546, C12Q 100, G01N 3348, G01N 33533, G01N 33554

Patent

active

049818031

ABSTRACT:
A reagent for reticulocyte counting by flow cytometry which comprises two solutions, namely, a stock solution for staining in which a dye is dissolved in a nonaqueous solvent, and a buffer solution which satisfies the optimum staining conditions.
By combining these two solutions immediately before measurement, a stable final staining solution for reticulocyte counting can always be obtained.

REFERENCES:
patent: 4288543 (1981-09-01), Sielaff et al.
patent: 4835101 (1989-05-01), Kao et al.
patent: 4835103 (1989-05-01), Cercek et al.
patent: 4842646 (1989-06-01), Gamblin
Walter C. Holmes and J. Ferris Darling, "The Hydrolysis of Auramine", Journal of the American Chemical Society, 46, 2343-2348 (1924).

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