Reagent for measuring amylase activity and measuring method ther

Details Reagent for measuring amylase activity and measuring method ther Reagent for measuring amylase activity and measuring method ther

1986-05-09

1989-03-14

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 15, 435 22, C12Q 154, C12Q 140

Patent

active

048123986

ABSTRACT:
Disclosed is a reagent for measuring amylase activity in body fluids by cleaving an oligosaccharide having a defined chain length with amylase in body fluids to produce a glucose and measuring said glucose, characterized in that the reagent is divided to two portions, the first portion comprises an enzyme for converting a glucose and/or maltose naturally present in body fluids to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate, and the second portion comprises said oligosaccharide being used as a substrate and a phosphoric acid ester of saccharides and/or saccharic acids; and a method for measuring amylase activity in body fluids comprising;

REFERENCES:
patent: 3879263 (1975-04-01), Adams
patent: 4169765 (1979-10-01), Keyes
Biochemistry (Second Ed.), Chapt. 16, part 2, Albert Lehninger Worth Publishers.
Clinical Chemistry, vol. 27, No. 6, pp. 806-815. "Suitability of Control Materials for Determination of .alpha.-Amylase Activity".

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