Reagent for measuring alanine aminotransferase activity

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

Reexamination Certificate

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Details

C435S026000

Reexamination Certificate

active

10507105

ABSTRACT:
A reagent for measuring an alanine aminotransferase activity comprising L-alanine, 2-oxoglutaric acid, lactate dehydrogenase, and reduced nicotinamide adenine dinucleotide, characterized by further comprising a substance having an activity of inhibiting lactate dehydrogenase activity is disclosed. Further, a method for measuring an alanine aminotransferase activity, characterized by bringing a sample to be analyzed, which may contain alanine aminotransferase, into contact with L-alanine, 2-oxoglutaric acid, lactate dehydrogenase, reduced nicotinamide adenine dinucleotide, and a substance having an activity of inhibiting a lactate dehydrogenase activity is disclosed. According to the reagent and method, an increase in the reagent blank reaction, i.e., an increase in the initial absorbance, can be suppressed.

REFERENCES:
patent: 4241179 (1980-12-01), Madappally et al.
patent: 5589348 (1996-12-01), Kaufman et al.
patent: 0 133 064 (1985-02-01), None
patent: 1 083 235 (2001-03-01), None
patent: 1083235 (2001-03-01), None
patent: 9-070299 (1997-03-01), None

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