Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1993-09-14
1996-08-27
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435179, 435180, 435 18, C12Q 137, C12Q 134, C12N 1108, C12N 1112
Patent
active
055500307
ABSTRACT:
Disclosed are a reagent for endotoxin-specific assay which comprises an insoluble carrier having immobilized thereon at least an endotoxin-sensitive factor derived from a limulus amebocyte; a kit for endotoxin-specific assay containing said reagent and a substrate for activated factor C or a substrate for clotting enzyme; a method for assaying endotoxin comprising applying a sample solution to said reagent to cause endotoxin in the sample to react with factor C in said reagent and determining a change of a substrate; and a process for preparing said reagent which comprises physically or chemically immobilizing at least an endotoxin-sensitive factor derived from a limulus amebocyte on an insoluble carrier. Endotoxin in a sample, even turbid or colored, can be specifically assayed with ease and rapidness without the influence of a (1.fwdarw.3)-.beta.-glucan.
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R. E. Bryant et al, Endotoxin Contamination of Enzyme Conjugates Used in Enzyme-Linked Immunosorbent Assays, J. Clin. Microbiol., 17, 1050-1053 (1983).
T. Nakamura, T. Morita and S. Iwanaga, Lipopolysaccharide-sensitive Serine-protease Zymogen (Factor C) Found in Limulus Hemocytes, European Journal of Biochemistry, vol. 154, 1986, pp. 511-521.
T. Obayashi et al., A New Chromogenic Endotoxin-Specific Assay Using Recombined Limulus Congulation Enzymes and its Clinical Applications, Clinica Chimica Acta, vol. 149, No. 1, 1985, pp. 55-65.
S. Nakamura et al, A Clottable Protein (Coagulation) from Amoebocyte Lysate of Japanese Horseshoe Crab (Tachypleus tridentatus, J. Biochem, vol. 80, No. 5, 1976, pp. 1011-1021.
Ohki Makoto
Tamura Hiroshi
Tanaka Shigenori
Carroll, II Lawrence J.
Mosher Mary E.
Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)
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