Reagent for determining .gamma.-glutamyl transpeptidase activity

Details Reagent for determining .gamma.-glutamyl transpeptidase activity Reagent for determining .gamma.-glutamyl transpeptidase activity

1994-03-22

1995-12-12

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 4, 435 24, 435 16, C12Q 148, C12Q 100, C12Q 137, C12Q 152

Patent

active

054749069

ABSTRACT:
A reagent for determination of .gamma.-GTP activity containing L-.gamma.-glutamyl-3-carboxy-4-nitroanilide or a salt as a substrate and a transition metal ion or salt, a method for stabilizing L-.gamma.-glutamyl-3-carboxy-4-nitroanilide using a transition metal ion salt, and a method for determining .gamma.-GTP activity using this reagent. The transition metal ion or salt effectively prevents non-enzymatic decomposition (hydrolysis) of the substrate, thereby making it possible to supply the reagent as a solution.

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