Reagent for Determining .alpha.-amylase activity and method for

Details Reagent for Determining .alpha.-amylase activity and method for Reagent for Determining .alpha.-amylase activity and method for

1993-11-04

1995-02-28

Russel, Jeffrey E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435201, C12Q 140, C12N 926

Patent

active

053936604

ABSTRACT:
A reagent for determining a-amylase activity, comprising a maltooligosaccharide derivative of the following formula ##STR1## (wherein either one of R.sub.1 and R.sub.2 is .beta.-galactopyranosil and the other is hydrogen, R.sub.3 is a group bonded to the reducing terminal glucose via a bond cleavable by .alpha.-amylase, which becomes a measurable substance upon cleavage of the bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes; and a method for determining .alpha.-amylase activity which comprises use of the reagent. The reagent of the present invention does not require use of any adjuvant enzyme and is stable since the substrate is not exposed to the decomposition by an adjuvant enzyme. The substrate used in the present invention has high affinity for .alpha.-amylase. Thus, the reagent and the determination method of the present invention make it possible to determine .alpha.-amylase activity with high sensitivity.

REFERENCES:
patent: 5158872 (1992-10-01), Chavez
Nippon Nogeikagaki Kaisha, vol. 65, No. 3, p. 117, dated Mar. 15, 1991 and English Summary.
Ogawa et al., "Differential Assay of Human Pancreatic & Salivary .alpha.-Amylase w/p-Nitrophenyl 6.sup.5 -O-.beta.-D-Galacopyrasoyl-.alpha.-maltopentaoside as the Substrate", Biosci., Biotech. Biochem. 56(12) 1933-1936, 1992.
Ishimaru et al., "Enzymatic Synthesis of 2-Chloro-4-nitrophenyl 4,6-O-3-Ketobutylidene .beta.-Maltopentaoside, a Substrate for .alpha.-Amylase", Biosci., Biotech., Biochem. 56(10), 1552-1556, 1992.

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