Reagent for determination of calcium and determination method

Chemistry: analytical and immunological testing – Metal or metal containing – Li – na – k – rb – cs – fr – be – mg – ca – sr – ba – ra

Reexamination Certificate

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C436S166000

Reexamination Certificate

active

07544516

ABSTRACT:
A method for determination of calcium in a sample derived from a living body, by the reaction of calcium in the sample with Chlorophosphonazo-III or a compound analogous thereto in the presence of vanadate ions, followed by determination of calcium in the sample on the basis of an optical change caused by the reaction product, is excellent in various respects as follows: it is free from the problem of absorption of carbonic acid gas caused by a high pH; it is free from the problem of use of a toxic reagent containing arsenic; it makes it possible to subject a large number of samples to determination in a short time because it can be applied to an autoanalyzer; and it permits determination in a wide range because of low sample blank values.

REFERENCES:
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patent: 6-505560 (1994-06-01), None
European Search Report dated Jun. 19, 2007.
Lizhong et al. “Studies on the Chromogenic Reaction of Mixed Surfactant System Vanadium (IV)—Chlorophosphonazo III-CTMAB-Emulsifier OP and its Application” Journal of Zhejiang Institute of Technology 1991, 1, 54-59.
Medda et al. “The Vanadate Complex of teh Calcium-Transport ATPase of teh Sarcoplasmic reticulum, its Formation and Dissolution” European Journal of Biochemistry 1983, 137, 7-14.
Qureshi, “The Spectrophotometric Determination of Calcium (II) in Steel Making Alloys”. Pakistan Journal of Science (1979), 31(3-6), 212-14.
Zagidullina et al. “Spectrophotometric Determination of the Acetic Acid Content”, Kemerovski Technological Institute of the Food Industry, Nos. 1-3, 1991, p. 230.
Lizhong et al. “Studies on the Chromogenic Reaction of Mixed Surfactant System Vanadium (IV)-Chlorophosphonazo III-CTAB-Emulsifier OP and Its Application”. Journal of Zhejiang of Technology, 1, 1991, p. 54-59.
Medda et al. “The vanadate complex of the calcium-transport ATPase of the sarcoplasmic reticulum, its formation and dissociation”. European Journal of Biochemistry, 1983, 137(1-2), 7-14.

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