Reagent for colorimetric determination of creative phosphokinase

Details Reagent for colorimetric determination of creative phosphokinase Reagent for colorimetric determination of creative phosphokinase

1979-04-05

1981-01-27

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 14, 435 15, 435 26, 435188, 435189, 435194, C12Q 150, C12N 996

Patent

active

042476330

ABSTRACT:
An all-in-one reagent is provided for the quantitative colorimetric determination of creatine phosphokinase in blood serum or plasma wherein the tetrazolium dye, 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT), preferably together with an electron transport such as diaphorase, and compatible substances which are enzymatically converted by creatine phosphokinase to an end product that will reduce said dye from its normally colorless state to a colored reduced state are combined into a homogeneous composition which can be dried to a stable reagent.

REFERENCES:
patent: 3540984 (1970-11-01), Deutsch
patent: 3764478 (1973-10-01), Bergmeyer et al.
patent: 3791931 (1974-02-01), Thum et al.
patent: 3929580 (1975-12-01), Forgione et al.
patent: 4012286 (1977-03-01), Sanderson et al.
Arigad et al., Reduced Nicotinamide Dinucleotide Phosphate Diaphorase from Bacillus subtilis, European J. Biochem., vol. 1, 1967 (pp. 102-109).
Warren, W. A., Activation of Serum Creatine Kinase by Dithiothreitol, Clinical Chemistry, vol. 18, No. 5, 1972 (pp. 473-475).

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