Reagent for calcium ion level determination

Details Reagent for calcium ion level determination Reagent for calcium ion level determination

1993-04-29

1999-05-11

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C12Q1/42

Patent

active

059027304

ABSTRACT:
A reagent for calcium ion level determination comprising a phospholipid, phospholipase D, choline oxidase, a surfactant and a divalent metal salt. The reagent can easily be prepared and with which quantitative determination of calcium ion can be made over a broad range of a calcium ion concentration with ease and accuracy in a continuous manner.

REFERENCES:
patent: 4223090 (1980-09-01), Mazza
European Search Report.
Patent Abstracts of Japan, vol. 12, No. 47 (C-475)(2894) Feb. 12, 1988 & JP-A-62 195 297.
(Unitika Ltd) Aug. 28, 1987.
Fresenius Zeitschrift fur Analytische Chemie, vol. 308, No. 1, Aug.1, 1982, Berlin Brd, pp. 17-20.
M. Sugawara et al.: "Surface tension titration of metal ions by using metal salts of fatty acids as surface-active indicators".
Murachi, T. et al. Reagents Containing Lecithin in Chem. Abs. 108: 71690, 1988.
Kido, T et al. Use of Phospholipase D in Chem. Abs. 105: 13027, 1986.

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