Reagent for assaying hydrogen peroxide and method of quantitativ

Details Reagent for assaying hydrogen peroxide and method of quantitativ Reagent for assaying hydrogen peroxide and method of quantitativ

1986-05-09

1989-08-01

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 7, 435 25, 435805, 435810, C12Q 128, C12Q 126, G01N 33535

Patent

active

048533289

ABSTRACT:
A reagent suitable for assaying a very small amount of hydrogen peroxide is composed of a moiety having a residue formed by removing one hydrogen from an active methylene group or an active methine group and a fluorescing moiety.
Hydrogen peroxide contained in a sample liquid is determined by reacting the sample with the foregoing reagent, a hydrogen donor, and peroxidase and measuring the intensity of the fluorescence emitted from the fluorescing material formed by the reaction.

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March Advanced Org. Chem. Reactions, Mechanisms and Structure McGraw-Hill Book Co. 1977.
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Weinstein et al., Chemical Abstracts, 92:22337s, p. 668-669 (1980), "Synthesis of Quaiacylglycol and Glycerol-.beta.-O-(.beta.-Methylumbilliferyl)ethers:lignin Model Substrates for Possible Fluorometric Assay of .beta.-Etherases".
Barman, Enzyme Handbook, vol. I, Springer-Verlag, New York, NY., 234-235 (1969).

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