Reagent for assaying creatine kinase

Details Reagent for assaying creatine kinase Reagent for assaying creatine kinase

1985-10-04

1988-04-26

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 17, 435 26, 435810, 435 14, C12Q 148, C12Q 150, C12Q 126, C12Q 154

Patent

active

047404582

ABSTRACT:
A reagent system for assaying creatine kinase is disclosed. The reagent system consisting essentially of a first reagent comprising glucose-6-phosphate dehydrogenase, .beta.-nicotinamideadenine dinucleotide (phosphate), and adenosine diphosphate, and a second reagent comprising creatine phosphate, said second reagent being maintained at a pH of from 7.5 to 10, and at least one of said first reagent and said second reagent containing glucokinase and glucose. The creatine kinase-assaying reagent exhibits remarkably improved stability in a dissolved state so that it can be prepared in large quantities and can be conventionally utilized to cope with urgent clinical examinations.

REFERENCES:
patent: 4250254 (1981-02-01), Modrovich
patent: 4277562 (1981-07-01), Modrovich
patent: 4438199 (1984-03-01), Miwa et al.

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