Reagent for analyzing leucocytes

Details Reagent for analyzing leucocytes Reagent for analyzing leucocytes

1994-12-20

1997-04-08

Housel, James C.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Preparation composition

436 63, 435 2, 435 725, 424533, 424534, G01N 3352

Patent

active

056187335

ABSTRACT:
A reagent for analyzing leucocytes comprises (a) at least one ionic surfactant, being either a cationic or an amphoteric surfactant, in an amount sufficient for lysing erythrocytes and causing damage to a part of cell membranes of leucocytes; (b) at least one organic compound having a hydrophobic group and an acidic group which has a negative charge in an aqueous solution in an amount sufficient for a preserving leucocyte morphology by combining with a cationic component in leucocytes; (c) a nonionic surfactant; and (d) a buffer for adjusting pH.

REFERENCES:
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patent: 5155044 (1992-10-01), Ledis et al.
patent: 5175109 (1992-12-01), Sakata et al.
patent: 5179026 (1993-01-01), Matsuda et al.
patent: 5196346 (1993-03-01), Lefevre et al.
patent: 5227304 (1993-07-01), Wong
patent: 5242832 (1993-09-01), Sakata
patent: 5250437 (1993-10-01), Toda et al.
patent: 5250438 (1993-10-01), Ryan
patent: 5350695 (1994-09-01), Colella et al.
patent: 5378633 (1995-01-01), von Behrens et al.
Patent Abstracts of Japan, vol. 15, No. 144 (P-1189).

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