Reagent compositions for use in sphering cells

Details Reagent compositions for use in sphering cells Reagent compositions for use in sphering cells

1994-12-22

1997-05-27

Wityshyn, Michael G.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Preparation composition

436 18, 436 63, 435 11, G01N 3350

Patent

active

056331673

ABSTRACT:
Methods for blood sample preparation using reagent compositions which include a zwitterionic surfactant for sphering of cells to eliminate orientational noise. When the reagent composition including a dye for staining a subset of cells is reacted with a blood sample, and the reaction mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the stained cells can be distinguished from the unstained cells, and when the cells are reticulocytes and mature erythrocytes, respectively, the volume and hemoglobin concentration of each reticulocyte or erythrocyte, and the hemoglobin content, mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

REFERENCES:
patent: 3684377 (1972-08-01), Adams et al.
patent: 3883247 (1975-05-01), Adams
patent: 4325706 (1982-04-01), Gershman et al.
patent: 4336029 (1982-06-01), Natale
patent: 4412004 (1983-10-01), Ornstein et al.
patent: 4490353 (1984-12-01), Crawford et al.
patent: 4575490 (1986-03-01), Ornstein et al.
patent: 4652517 (1987-03-01), Scholl et al.
patent: 4707451 (1987-11-01), Sage, Jr.
patent: 4735504 (1988-04-01), Tycko
patent: 4853338 (1989-08-01), Benezra et al.
patent: 4883867 (1989-11-01), Lee et al.
patent: 4971917 (1990-11-01), Kuroda
patent: 4981803 (1991-01-01), Kuroda
patent: 4985174 (1991-01-01), Kuroda et al.
patent: 5039613 (1991-08-01), Matsuda et al.
patent: 5075556 (1991-12-01), Fan et al.
patent: 5242832 (1993-09-01), Sakata
Cytometry, vol. 7 (1986) "Flow Cytometry of DNA Content Using Oxazine 750 or Related Laser Dyes with 633 nm Excitation", Shapiro, et al., pp.107-110.
Analytical Cytology, 2nd Ed., "The Photometric Chemical Analysis of Cell", in Mellors, R.C., Ed., Pollister A.W. and Ornstein L., McGraw-Hill, New York (1959) pp. 431-518.

Affiliated with

Also associated with

Rating

Reagent compositions for use in sphering cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Reagent compositions for use in sphering cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent compositions for use in sphering cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2328545